cell lines human lung fibroblast cell line  (Bio-Rad)

Journal: bioRxiv

Article Title: A StayGold-based calcium ion indicator

doi: 10.64898/2026.03.06.710044

Figure Lengend Snippet: Mammalian cell-based characterization of HiCaRI ( A ) Image of HeLa cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).

Article Snippet: The HeLa cell line was purchased from ATCC (#CCL-2).

Techniques: Cell Characterization, Expressing, Fluorescence, Comparison, Transfection

Journal: bioRxiv

Article Title: Towards molecular-based functional classification of fetal bovine serum

doi: 10.64898/2026.03.16.712020

Figure Lengend Snippet: (A) Hierarchical clustering dendrograms of FBS conditions based on differential gene expression profiles in MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Clustering was performed using the number of differentially expressed genes (DEGs; |log2FC| > 0.6, adjusted p-value < 0.05) as a distance metric. Horizontal red lines indicate the clustering threshold of 100 DEGs, defining distinct FBS clusters. MRC-5 and THP-1 cells show 4 distinct clusters, while Jurkat cells display 5 clusters, revealing cell type-specific responses to FBS alternatives. (B) Module size distributions from WGCNA for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Bar colors represent distinct co-expression modules identified using signed networks with Pearson correlation (soft-thresholding power selected to achieve scale-free topology R ≥ 0.8; minimum module size = 30 genes; merge cut height = 0.25). MRC-5 cells: 7 modules (turquoise: 4,728 genes; blue: 3,580; brown: 2,417; grey: 936; yellow: 634; green: 447; red: 234). Jurkat cells: 7 modules (turquoise: 4,234; blue: 3,635; grey: 2,289; brown: 1,464; yellow: 660; green: 240; red: 204). THP-1 cells: 5 modules (turquoise: 4,201; blue: 4,16; grey: 2,478; brown: 2,184; yellow: 434). (C) Module-trait relationship heatmaps showing Pearson correlations between module eigengenes and FBS cluster assignments for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Each cell displays the correlation coefficient (color scale: blue = -0.5, white = 0, red = 0.5) and corresponding p-value. Significant correlations (p < 0.05, calculated using corPvalueStudent) indicate modules with expression patterns specifically associated with FBS clusters. Notable associations include: MRC-5 turquoise module with Cluster 1 (r = - 0.45, p = 3.22×10 ); Jurkat red module with Cluster 2 (r = 0.76, p = 1.38×10 ); THP-1 yellow module with Cluster 1 (r = 0.65, p = 2.54×10 8).

Article Snippet: The adherent human lung fibroblast cell line MRC-5 (CCL-171), the suspension human monocytic cell line THP-1 (TIB-202), and the human acute T-cell leukemic cell line Jurkat Clone E6-1 (TIB-152) were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Gene Expression, Expressing

Journal: Microbial Cell Factories

Article Title: A novel green synthesized ZnO-based antimicrobial nanocomposite: synergistic action, in vitro cytotoxicity, and molecular docking studies of ceftazidime, metformin, and chitosan against multidrug-resistant Salmonella enterica

doi: 10.1186/s12934-026-02934-x

Figure Lengend Snippet: Dose-dependent cytotoxic effect of ZnO/CS/CAZ/MTF nanocomposite on WI-38 lung fibroblast cells determined via MTT assay

Article Snippet: The human lung fibroblast cell line (WI-38) was obtained from ATCC through the Holding company for biological products and vaccines (VACSERA) in Cairo, Egypt.

Techniques: MTT Assay